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A relatively large number of biocides are used in hospitals and day-care centres. In a previous study, we showed that ammonia and formaldehyde inhibited the nasal immune response to nasal influenza virus in man. Here we investigate the effect of some biocides on nasal immune response to inactivated influenza virus. In a double-blind study, 66 healthy adult subjects (42 men and 24 women), aged 22 to 74 years, were randomly assigned to seven groups of 11 each. Each subject inhaled 3.85 x 10(9) TCID(50) of inactivated influenza virus twice in the nasal cavity on separate days during the experiment. Subjects were randomly assigned to one of seven biocides or to negative control. Two subgroups were assigned to two different dose levels of each biocide. Subjects were given four visits during the experiment. On visit 1, subjects received one of the biocides at a low dose (0.9 microL per nostril, approximately 1.15 x 10(7) TCID(50)), and on visit 2 they received the biocides at a high dose (5.4 microL per nostril, approximately 1.5 x 10(8) TCID(50)). On visit 3, subjects inhaled only virus without treatment; on visit 4 they received a placebo or the biocides again. Nasal washes were obtained on visits 2 and 4 and analysed for IgA, IgG, and IgM antibodies to the virus. The following biocides, irrespective of dose, were investigated: p-cresol, benzalkonium chloride, chlorphenesin, cetrimide, dimethyl-glutaronitrile (nitromersol), and 2-(4-chloro-phenyl)-2-methyl-propan-1-ol (chlorobenzol). Repeated inhalation of biocides induced no impairment of nasal immune response to the virus. We conclude that repeated inhalation of biocides, according to
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