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usefulness of the ampliseq cancer panel in screening of microsatellite instability in patients with tissue-based diagnosis of colorectal cancer. the cancer panel ampliseq tm cancer (cgp), based on next-generation sequencing technology, covers 100 genes and can be easily applied to formalin-fixed paraffin-embedded tissue. several studies have shown the usefulness of this assay for predicting dna mismatch repair (mmr) status, but the results obtained by the cgp remain controversial. to assess the usefulness of the cgp for predicting microsatellite instability (msi) status in patients with colorectal cancer (crc), based on histology. tumor specimens were stained with immunohistochemistry using five monoclonal antibodies (mlh1, pms2, msh2, msh6, and epcam) to detect mmr proteins. the cgp was performed in the initial diagnosis of 31 consecutive cases of crc. the overall concordance rate for assessing msi status by using the cgp was 71.0% (22/31) compared with pathological msi detection in 81.0% (26/31) of samples. the cgp was able to differentiate between specimens with and without msi in 20 samples (64.5%). when we considered the msi status by using the cgp, crc patients (13 of 13 [100%]) with msi-h tended to have a significantly better prognosis than that with msi-l or mss by using the cgp (log-rank p = 0.007). no msi-h tumor was identified in crc cases without mmr protein expression. in addition, this result may be consistent with the better 5-year survival rate of crc patients with msi-h. these findings may support the use of this cgp as a primary screening test for detecting msi in crc cases.by staff | jul 8, 2014 5:28 am (1) comment | commenting has been closed | e-mail the author posted to: legal writes, legal writes a connecticut man was sentenced to 20 years in prison for his role in an elaborate insurance scam that targeted an unsuspecting family, according to the u.s. attorney’s office in new haven. david diaz, 35, pleaded guilty to two counts of conspiring to commit mail fraud and was sentenced by u. district judge janet bond arterton on monday. the charges stemmed from a scheme in which diaz and two co-conspirators allegedly convinced a woman’s family, including her father, to have their home insured for a total of $1.6 million. prosecutors say the three conspirators convinced the family to accept a 30-year fixed-rate mortgage on the home that it already owned, claiming the mortgage would be cheaper than the original loan. then, the conspirators persuaded the family to insure the home at a cost of more than $500,000, according to the u. attorney’s office. the insurance coverage was allegedly paid for by a gofundme online fundraising website, according to the u. diaz allegedly took money from the gofundme account and then transferred funds out of the account to the family’s bank account and to three different individuals, according to the u.
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