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solidworks activator 2016 is to be able to activate the solidworks activator 2016 software so that you can use it freely without signing in.Ontogeny and cyclooxygenase regulation of prostaglandin synthesis in rat serosal mesothelium. Mammalian alveoli undergo a marked remodeling process after birth, including a transition from a low-resistance (LR) to a high-resistance (HR) pulmonary gas exchange membrane and a change from a predominantly proliferative to a predominantly secretory type of mesothelium. The physiological significance of these changes has not been fully elucidated, but they may reflect essential changes in lung function. The cytoprotective mediators synthesized by mesothelial cells are prostaglandins (PGs), and two PG synthase isozymes, cyclooxygenase (COX) 1 and COX2, are essential for fetal lung development, as well as for maintenance of fetal pulmonary function. We aimed to determine the developmental profiles of these PG synthases, their regulation by inflammatory mediators, and the PG synthetic capacity of rat serosal mesothelium in vitro. By real-time reverse transcription-polymerase chain reaction (RT-PCR), COX1 and COX2 messenger RNAs (mRNAs) were detected in fetal rats at gestational day (GD) 17 and day of birth (D0), i.e., prior to birth, in response to the proinflammatory mediators tumor necrosis factor-alpha, interleukin (IL)-1beta, and lipopolysaccharide, and in cultured rat serosal mesothelium. Prostaglandin (PG) synthesis, measured as formation of an immunoactive PG metabolite after [3H]arachidonic acid (AA) incubation, was higher at D0 than at GD17 in fetal rats, indicating that, from GD17 onward, a PG synthase other than COX1 is functionally expressed in rat serosal mesothelium. Tumor necrosis factor-alpha, IL-1beta, or lipopolysaccharide increased PG synthesis from GD17 to D0, with a more pronounced effect on PG synthesis at D0 than on PG synthesis at GD17. Morphology-independent PG synthesis was detectable in cultured rat serosal mesothelium. Both COX1 and COX2 mRNAs were detected in cultured rat serosal mesothelium 1cdb36666d
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